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Studies on lectins. XLVII. Some properties of D-galactose binding lectins isolated from the seeds of Butea frondosa, Erythrina indica and Momordica charantia. 1980

V Horejsí, and M Tichá, and J Novotný, and J Kocourek

The lectins from the seeds of Butea frondosa, Erythrina indica and Momordica charantia were isolated by affinity chromatography on O-alpha-D-galactosyl polyacrylamide gels. The Butea frondosa lectin has sedimentation coefficient S20,W = 6.7 S, its molecular weight is 141 000 and it consists of noncovalently associated subunits of molecular weights 36 000 and 65 000. The lectin contains two 1/2-cystine and 3--4 methionine residues per molecule, 4.3% neutral sugar and 0.5% glucosamine. Threonine is the single N-terminal amino acid residue. The lectin from Erythrina indica seeds has a sedimentation coefficient S20,W = 4.0 S, its molecular weight is 66 200 and it consists of two kinds of subunit (molecular weights 30 000 and 34 000, respectively), which are noncovalently associated. The lectin is devoid of cystein, has a neutral sugar content 5.6% and glucosamine content 0.46%. It contains probably one Mn atom per subunit. The N-terminal amino acid sequence is Val-Glu-Val-Leu-(Phe)-Phe-(Ala)-Phe-. The lectin from Momordica charantia seeds has a sedimentation coefficient S20,W = 6.5 S, its molecular weight is 129 000. It interacts weakly with Sephadex G-200. The lectin consists of subunits of molecular weights 29 000, 32 000 and 36 000, respectively, held together by cystine bridges. Its content of cystine and methionine residues is relatively high, 4.1% neutral sugar and 0.5% glucosamine form the carbohydrate moiety. Two N-terminal amino acids (asparagine and valine) are present.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002241 Carbohydrates A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrate
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D005690 Galactose An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood. D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino
D012639 Seeds The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield. Diaspores,Elaiosomes,Embryos, Plant,Plant Embryos,Plant Zygotes,Zygotes, Plant,Diaspore,Elaiosome,Embryo, Plant,Plant Embryo,Plant Zygote,Seed,Zygote, Plant
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D037102 Lectins Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition. Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal

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