-transparent.png){kind=logo}
A new type of sorbents for affinity chromatography is suggested and used to purify tRNA methylases. tRNA was immobilized on aminooxybutylcellulose via the oxidized 3'-end. In order to bind other enzymes specific for nucleic acids in general, e. g. nucleases, and to achieve a higher degree of purification the crude enzyme preparation was treated with rRNA immobilized on aminooxybutycellulose. The sequential application of two sorbents mentioned allows to get an approximately two hundred fold purification of total tRNA methylases. In a separate experiment the possibility of individual tRNA methylase fractionation by means of elution with a NACl gradient was shown; the degree of purification for some methylases was more than a thousand fold.
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
October 1993,
Biochemistry and molecular biology international,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
July 1995,
Analytical biochemistry,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
September 2004,
Journal of chromatography. A,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
July 1986,
Biochemical and biophysical research communications,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
April 2000,
Biotechnology and bioengineering,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
January 1980,
Analytical biochemistry,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
December 1977,
Thrombosis research,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
June 2016,
Journal of separation science,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
January 1974,
Biochimie,
A A Nedospasov, and
R M Khomutov, and
A S Gambarian, and
I A Morozov, and
T V Venkstern
December 1980,
The Journal of biological chemistry,
