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Multinucleate cells in cultures of human amniotic fluid form by fusion. 1980

R E Priest, and J H Priest, and C H Laundon, and P W Snider

The major class of cells subcultured from human amniotic fluid retains properties of trophoblast. The objective of this study was to determine whether the multinucleate cells appearing in these cultures form by fusion, as is true for syncytiotrophoblast. Culture methodology involved subdivision of one flask into two, one of which was labeled with 3H-thymidine and the other with 14C-thymidine. Aliquots of each labeled culture were mixed together and allowed to grow. Autoradiographic study revealed binucleate cells containing one nucleus labeled with 3H and the other with 14C. This result indicates that fusion is one means by which binucleate cells form. The distribution of labeled and unlabeled nuclei in 217 binucleate cells obtained in two experiments was then determined. The distribution conformed to that predicted from the assumption that fusion was random and the only way for binucleate cells to form. We conclude that the binucleate cells appearing in cultures of human amniotic fluid form by fusion, as do the multinucleate cells in trophoblast. Our results suggest further that fusion is the sole means by which binucleate cells arise.

UI MeSH Term Description Entries
D002459 Cell Fusion Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization. Cell Fusions,Fusion, Cell,Fusions, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000653 Amniotic Fluid A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS). Amniotic Fluid Index,Amniotic Fluid Indices,Amniotic Fluids,Fluid Index, Amniotic,Fluid Indices, Amniotic,Fluid, Amniotic,Fluids, Amniotic,Index, Amniotic Fluid,Indices, Amniotic Fluid
D001345 Autoradiography The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed) Radioautography
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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