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The authors report of a process for producing high-purity fluorescein for injection purposes which is characterized by the fact that synthesis-induced by-products are separated from the raw fluorescein, obtained by any process, by means of a clean-up chain consisting in step-by-step monoacetylation in an aqueous-alkaline solution, subsequent diacetylation in pyridine or a pyridine-glacial acetic acid mixture, saponification of the diacetyl product by sodium methoxide in methanol, and precipitation of the fluorescein with anhydrous formic acid.
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