-transparent.png){kind=logo}
A sensitive and reliable high-pressure liquid chromatography (HPLC) assay for miloxacin and its two principal metabolites, 5,8-dihydro-8-oxo-2H-1,3-dioxolo[4,5-g]quinoline-7-carboxylic acid (M-1) and 1,4-dihydro-1,6-dimethoxy-7-hydroxy-4-oxoquinoline-3-carboxylic acid (M-2), in human serum and urine was developed. A strong anion-exchange Zipax SAX column using a mobile phase of 0.01 M citric acid solution containing 0.03 M sodium nitrate with pH 5.0 was used to achieve separation of the three compounds. The retention times of miloxacin, M-1, and M-2 were 3.8, 9.3, and 5.9 min, respectively. Serum and urine concentrations of these compounds as low as 10 ng/ml were measured. When results from the HPLC assay were compared with those from the microbiological assay of serum and urine samples from human subjects receiving miloxacin orally, the correlation coefficients were 0.94 for the serum and 0.99 for the urine. The HPLC assay method presents an alternative to the microbiological assay and permits future pharmacokinetic investigations of miloxacin.
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
October 1974,
The Journal of laboratory and clinical medicine,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
January 1979,
Journal of chromatography,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
October 1976,
Biochemical medicine,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
February 1979,
Clinica chimica acta; international journal of clinical chemistry,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
June 1995,
Therapeutic drug monitoring,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
December 1985,
Pharmaceutisch weekblad. Scientific edition,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
January 1980,
Journal of chromatography,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
July 1984,
Journal of chromatography,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
August 1979,
Journal of pharmaceutical sciences,
A Yoshitake, and
K Kawahara, and
F Shono, and
I Umeda, and
A Izawa, and
T Komatsu
December 1980,
Journal of pharmaceutical sciences,
