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Fluorescence induction at -196 degrees C has been monitored in chloroplasts rapidly frozen after poising at different redox potentials at room temperature. It was found that, as at room temperature, the initial level of fluorescence observed upon shutter opening (Fo), relative to the final level observed after 10 seconds of illumination (Fm) increased as the redox potential of the chloroplasts was lowered. Redox titration and revealed the presence of two quenching components with Em, 7.8 at -70 mV and -275 mV accounting for approx. 75% and 25% of the variable fluorescence (Fv). Parallel observation of fluorescence yield at room temperature similarly gave two components, with Em, 7.8 at -95 mV and -290 mV, also accounting for appox. 75% and 25%. Simultaneous measurement of fluorescence emission at -196 degrees C at 695 nm and 735 nm indicated that both emissions are quenched by the same redox components.
P Horton, and
N R Baker
January 1976,
Biofizika,
P Horton, and
N R Baker
January 1977,
Biofizika,
P Horton, and
N R Baker
November 1977,
Biochimica et biophysica acta,
P Horton, and
N R Baker
May 1970,
Doklady Akademii nauk SSSR,
P Horton, and
N R Baker
January 1979,
Biochimica et biophysica acta,
P Horton, and
N R Baker
January 1976,
Biofizika,
P Horton, and
N R Baker
January 1985,
Doklady Akademii nauk SSSR,
P Horton, and
N R Baker
January 2003,
Photochemistry and photobiology,
P Horton, and
N R Baker
January 1978,
Archivum immunologiae et therapiae experimentalis,
