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The interaction between rhodopsin SH-groups in the photoreceptor membrane suspension with different titrating reagents (Ag HOGM, DTNB) was studied. After rhodopsin bleaching the number of free SH-groups increased from two in the dark to six after illumination. Such increase of SH-groups number was observed only by the amperometric titration procedure. The influence of the "dark" and "light exposed" SH-group modifications on regeneration ability of rhodopsin in the presence of 11-cis-retinal was investigated. No dependence of regeneration on SH-groups modification in the dark was observed, while modification of "light" SH-groups decreases rhodopsin regeneration ability. The distribution of SH-groups in rhodopsin molecules and SH-groups dependence on protein conformation are discussed.
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
October 1966,
Biochimica et biophysica acta,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
January 1978,
Membrane biochemistry,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
October 1952,
Nutrition reviews,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
October 1978,
Biochemistry,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
January 1971,
Biofizika,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
November 1973,
Biochimica et biophysica acta,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
February 1974,
Nature,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
January 1976,
Zeitschrift fur Naturforschung. Section C, Biosciences,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
January 1978,
Uspekhi sovremennoi biologii,
G R Kalamkarov, and
L E Skokan, and
M A Ostrovskiĭ
May 1952,
The Journal of general physiology,
