Biomaterial Database

Reversed-phase high-performance liquid chromatographic separation and quantification of individual human bile acids. 1980

G Mingrone, and A V Greco

The reversed-phase high-performance liquid chromatographic separation and quantification of individual bile acids is described. Taurine- and glycine-conjugated bile acids were separated and detected directly by an ultraviolet absorbance detector operating at 200 nm. Simultaneous quantitation of at least 100 ng of each conjugated bile acid is possible. Carboxylic (free and glycine-conjugated) bile acids were esterified with p-bromophenacyl-bromide. The reaction, using N,N-diisopropylethylamine as catalyst, yields quantitatively the strongly absorbing p-bromophenacyl esters whch can be determined by absorbance measurement at 254 nm. Simultaneous quantitation of less than 20 ng of each bile acid is possible. The present method is applied to the quantitation of individual bile acids in ten human gallbladder bile samples.

UI	MeSH Term	Description	Entries
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004951	Esterification	The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in the presence of a trace of mineral acid as catalyst or the reaction of an acyl chloride with an alcohol. Esterification can also be accomplished by enzymatic processes.	Esterifications
D005021	Ethylamines	Derivatives of ethylamine (the structural formula CH3CH2NH2).
D005704	Gallbladder	A storage reservoir for BILE secretion. Gallbladder allows the delivery of bile acids at a high concentration and in a controlled manner, via the CYSTIC DUCT to the DUODENUM, for degradation of dietary lipid.	Gallbladders
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000098	Acetophenones	Derivatives of the simplest aromatic ketone acetophenone (of general formula C6H5C(O)CH3).
D001647	Bile Acids and Salts	Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.	Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry