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A high-performance liquid chromatographic method was developed for the simultaneous determination of phenylbutazone and its metabolites, oxyphenbutazone and gamma-hydroxy-phenylbutazone, in plasma and urine. Samples were acidified with hydrochloric acid and extracted with benzene--cyclohexane (1:1, v/v). The extract was redissolved in methanol and chromatographed on a muBondapak C18 column using a mobile phase of methanol--0.01 M sodium acetate buffer (pH 4.0) in a linear gradient (50 to 100% methanol at 5% min; flow-rate 2.0 ml/min) in a high-performance liquid chromatograph equipped with an ultraviolet absorbance detector (254 nm). The detection limit for phenylbutazone, oxyphenbutazone and for gamma-hydroxyphenylbutazone was 0.05 microgram/ml. A precise and sensitive assay for the determination of phenylbutazone and its metabolites was established.
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
May 1989,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
February 2003,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
April 2001,
Journal of chromatography. B, Biomedical sciences and applications,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
July 1984,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
May 1986,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
September 1989,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
December 1985,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
January 1994,
Biomedical chromatography : BMC,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
February 1984,
Journal of chromatography,
T Marunaka, and
T Shibata, and
Y Minami, and
Y Umeno
February 1996,
Journal of chromatography. B, Biomedical applications,
