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Comparison of "direct" and "indirect" zirconyl-phosphate technics for carcinoembryonic antigen determination in plasma from patients having benign or malignant disease. 1980

M Koch, and T A McPherson

A simplified "direct" zirconly-phosphate gel radioimmunoassay for carcinoembryonic antigen (CEA) is compared with the "indirect" method using reagents provided in the Roche¿ CEA kit. Statistical analysis shows that (1) the methods are comparable in sensitivity and specificity; (2) the values obtained are, in most case, interchangeable; (3) the results by the two methods are comparable both among patients who have colorectal cancer and among those who have other diseases. For general use, the direct assay has proven to be an adequate alternative to the indirect assay.

UI MeSH Term Description Entries
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002272 Carcinoembryonic Antigen A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment. Antigens, CD66e,CD66e Antigen,Antigen, CD66e,Antigen, Carcinoembryonic,CD66e Antigens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015040 Zirconium A rather rare metallic element with atomic number 40, atomic weight 91.224, and symbol Zr.

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