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Provided the intragastric pH is greater than 6 and the collected gastric juice is boiled and neutralised immediately, considerable amounts of gastrin-like immunoreactivity can be found in human gastric juice. Characterisation according to size and charge reveals that nearly all the gastrin-like immunoreactivity is similar to gastrin 1-17. Direct stimulation of the G-cells with protein solution is followed by a significant and simultaneous increase of acid secretion, serum gastrin, and gastrin output into the lumen of the stomach in control subjects and patients with duodenal ulcer.
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