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Proteoglycan synthesis and secretion by bovine aortic tissue in organ culture. 1980

P Vijayagopal, and B Radhakrishnamurthy, and S R Srinivasan, and J McMurtrey, and G S Berenson

The incorporation of 35S into proteoglycans (PG) of bovine aorta segments maintained in short term culture was investigated. The labeled PG were isolated by 4.0 M guanidinium chloride extraction and collagenase and elastase digestion and specific activities determined. The culture medium contained about 10% of the total uronate. In the sequential extraction of tissue PG, elastase treatment released the most uronate material. Guanidinium chloride extracted PG showed the highest degree of sulfation while the elastase solubilized PG had the lowest sulfate content. In the culture medium the highest specific activity was noted in heparan sulfate PG, followed by the chondroitin sulfates PG and then dermatan sulfate PG. Among the PG from tissue extracts, dermatan sulfate chains from guanidinium chloride extracted and collagenase solubilized PG showed similar specific activity. The highest specific activity of haparan sulfate was observed in the elastase solubilized PG. The specific activity of chondroitin sulfate chains was the lowest in all tissue PG preparations. These studies provide further evidence of the metabolic heterogeneity of aorta PG.

UI MeSH Term Description Entries
D009924 Organ Culture Techniques A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1) Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D011509 Proteoglycans Glycoproteins which have a very high polysaccharide content. Proteoglycan,Proteoglycan Type H
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D006025 Glycosaminoglycans Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE). Glycosaminoglycan,Mucopolysaccharides
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D001161 Arteriosclerosis Thickening and loss of elasticity of the walls of ARTERIES of all sizes. There are many forms classified by the types of lesions and arteries involved, such as ATHEROSCLEROSIS with fatty lesions in the ARTERIAL INTIMA of medium and large muscular arteries. Arterioscleroses
D013462 Sulfur Radioisotopes Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes. Radioisotopes, Sulfur

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