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An enzyme-linked immunosorbent assay (ELISA) for the quantitation of immune complexes in sera has been developed using Raji cells. In this assay the Raji cell-adherent complexes are detected by an alkaline phosphatase-conjugated rabbit anti-human IgG and the results are obtained by spectrophotometric measurements of the concentration of yellow colour produced after the enzyme's substrate, p-nitrophenyl phosphate, is added. This assay is as sensitive and reproducible as the Raji cell radioimmune assay using 125I, as it can detect as little as 16 micrograms of immune complex equivalent to heat-aggregated IgG/ml in serum samples. This method is inexpensive, convenient and most importantly, avoids the biohazards of using an isotope.
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
September 1983,
Analytical biochemistry,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
February 1980,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
January 1987,
The American journal of tropical medicine and hygiene,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
January 1982,
Research communications in chemical pathology and pharmacology,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
October 1983,
American journal of obstetrics and gynecology,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
March 1990,
Journal of immunological methods,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
January 1982,
Alcoholism, clinical and experimental research,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
August 1988,
Journal of clinical & laboratory immunology,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
December 1989,
Journal of immunological methods,
C Cunningham-Rundles, and
W E Brandeis, and
T Zacharczuk, and
R A Good, and
N K Day
September 1983,
American journal of veterinary research,
