-transparent.png){kind=logo}
Methods are described for isolating highly purified FSH from porcine pituitary glands. The biological activity of pure material was 22 NIH-FSH-P2 U/mg, as judged by the ovarian weight augmentation bioassay. Virtually no immunoreactive LH was indicated by RIA, and only one component was detected by both gel electrophoresis and immunoprecipitation. Amino acid and carbohydrate analyses of the highly purified FSH indicated the presence of 1-tryptophan, a high content of aspartic and glutamic acids, and 6% sialic acid. Molecular weights of untreated and guanidine-treated porcine FSH were estimated from hydrodynamic measurements of Stokes radii and sedimentation coefficients. A highly specific and sensitive RIA was also developed for this hormone.
R J Whitley, and
H T Keutmann, and
R J Ryan
March 1976,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
R J Whitley, and
H T Keutmann, and
R J Ryan
December 1967,
Biochemistry,
R J Whitley, and
H T Keutmann, and
R J Ryan
April 1971,
Biochemistry,
R J Whitley, and
H T Keutmann, and
R J Ryan
December 1971,
The Journal of biological chemistry,
R J Whitley, and
H T Keutmann, and
R J Ryan
December 1972,
Endocrinology,
R J Whitley, and
H T Keutmann, and
R J Ryan
January 1971,
Neuroendocrinology,
R J Whitley, and
H T Keutmann, and
R J Ryan
July 1971,
Biochemistry,
R J Whitley, and
H T Keutmann, and
R J Ryan
April 1969,
Endocrinology,
R J Whitley, and
H T Keutmann, and
R J Ryan
July 1992,
Nihon Sanka Fujinka Gakkai zasshi,
R J Whitley, and
H T Keutmann, and
R J Ryan
October 1964,
Biochimica et biophysica acta,
