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We purified human prostatic acid phosphatase (hPAP) from prostatic tissues by affinity chromatography, DEAE cellulose and gel filtration and also examined physicochemical properties of highly purified PAP. We developed a double-antibody radioimmunoassay for hPAP in serum, with use of antiserum raised in rabbit against highly purified PAP. The antiserum did not cross react with acid phosphatase from platelets and red blood cells. Experimental detail are outlined to assess the reproducibility and reliability of the method under various conditions. The upper limit of the serum PAP levels in the present assay was set at 3.0 ng/ml by 162 determinations of samples. The serum PAP levels of 2 untreated patients with prostatic carcinoma were higher than 3.0 ng/ml and 39 patients with benign prostatic hyperplasia were an average value of 1.9 ng/ml.
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
July 1981,
Kaku igaku. The Japanese journal of nuclear medicine,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
January 1982,
Annales de biologie clinique,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
April 1981,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
June 1981,
JAMA,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
February 1982,
Kaku igaku. The Japanese journal of nuclear medicine,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
December 1981,
Kaku igaku. The Japanese journal of nuclear medicine,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
December 1981,
Kaku igaku. The Japanese journal of nuclear medicine,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
March 1983,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
November 1980,
The Journal of urology,
J Morikawa, and
M Nakamura, and
K Mori, and
R Ohsawa, and
M Miki, and
T Machida
June 1980,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
