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A Bradley's modified procedure is described herein. It allows the diagnosis of acute viral hepatitis A by evidence of IgM anti HAV: after IgG anti-HAV have been preferentially absorbed with Staphylococcus aureus cells positive for protein A, we test for residual IgM anti-HAV in the supernatant (absorbed serum) by radioimmunoassay. We have documented the ability of this procedure to differentially detect IgM or IgG anti-HAV by analyzing 308 sera. This simple method clearly distinguishes between recent (28 cases) and past HAV infection which is very frequent in France: 80% in adults.
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