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After incubation of 1,3,5(10),16-oestratetraenol, a 16-dehydrosteroid, with rat liver microsomes, 16 alpha, 17 alpha-epoxy-oestratrienol was isolated as metabolite. The compound was detected by the use of mass fragmentography after purification of the incubation extract by thin-layer chromatography. Since the epoxide is rapidly hydrolysed by a hepatic epoxide hydratase, only very small concentrations of this metabolite were present in the incubation extract. When styrene oxide was added to the incubation mixture as inhibitor of the epoxide hydratase, the yield of the steroid epoxide increased considerably. Final identification of the oestrogen epoxide was performed by recording mass spectra and by comparison with authentic reference material.
L Siekmann, and
P Thull, and
H Breuer
September 1980,
Acta endocrinologica,
L Siekmann, and
P Thull, and
H Breuer
October 1980,
Journal of steroid biochemistry,
L Siekmann, and
P Thull, and
H Breuer
August 1968,
Steroids,
L Siekmann, and
P Thull, and
H Breuer
September 1961,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
L Siekmann, and
P Thull, and
H Breuer
March 1965,
Life sciences (1962),
L Siekmann, and
P Thull, and
H Breuer
November 1970,
European journal of biochemistry,
L Siekmann, and
P Thull, and
H Breuer
April 1995,
Lipids,
L Siekmann, and
P Thull, and
H Breuer
August 1968,
European journal of biochemistry,
L Siekmann, and
P Thull, and
H Breuer
January 1972,
Journal of the Chemical Society. Perkin transactions 1,
L Siekmann, and
P Thull, and
H Breuer
January 1984,
Annales de recherches veterinaires. Annals of veterinary research,
