Biomaterial Database

Effect of peptichemio and of peptichemio plus hydrocortisone on growth and alkaline phosphatase activity in HeLa cells in vitro. 1980

S D'Ancona, and M T Barberio, and P Adami, and R Poltronieri

The actions of Hydrocortisone, Peptichemio and of Peptichmio + Hydrocortisone mixture in HeLa cells have been experimented. The results show an increase of cell number in the controls and a lower, but not statistically different growth, in the Hydrocortisone-treated cultures. In the cultures treated with peptichemio the growth is hardly evident, in the ones treated with the Hydrocortisone + Peptichemio mixture, the cell number is decreasing with time. ALP activity in cells treated with drugs is much higher than in the controls ones. The results either on cellular growth and on ALP activity, are compared to the Ara C ones. No significant difference in media ALP activity between drug-treated cultures and controls is evidentiated. The results show in the Peptichemio + Hydrocortisone mixture, that the action of Hydrocortisone is overcome by the Peptichemio one. By the cytochemical technique the enzyme localization is particularly evident in the cytoplasmic inclusions, which are very numerous in the cells treated with Peptichemio.

UI	MeSH Term	Description	Entries
D008558	Melphalan	An alkylating nitrogen mustard that is used as an antineoplastic in the form of the levo isomer - MELPHALAN, the racemic mixture - MERPHALAN, and the dextro isomer - MEDPHALAN; toxic to bone marrow, but little vesicant action; potential carcinogen.	Medphalan,Merphalan,Phenylalanine Mustard,Sarcolysine,Sarkolysin,4-(Bis(2-chloroethyl)amino)phenylalanine,Alkeran,L-PAM,Mustard, Phenylalanine
D010440	Peptichemio	A mixture of six synthetic oligopeptides, each containing MELPHALAN. It is used as a broad-spectrum antineoplastic due to its alkylating and antimetabolic actions but, is toxic to bone marrow, gastrointestinal system and vasculature.
D004357	Drug Synergism	The action of a drug in promoting or enhancing the effectiveness of another drug.	Drug Potentiation,Drug Augmentation,Augmentation, Drug,Augmentations, Drug,Drug Augmentations,Drug Potentiations,Drug Synergisms,Potentiation, Drug,Potentiations, Drug,Synergism, Drug,Synergisms, Drug
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006854	Hydrocortisone	The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.	Cortef,Cortisol,Pregn-4-ene-3,20-dione, 11,17,21-trihydroxy-, (11beta)-,11-Epicortisol,Cortifair,Cortril,Epicortisol,Hydrocortisone, (11 alpha)-Isomer,Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer,11 Epicortisol
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.