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Three variants of pyruvate kinase are described which have marked reduction of activity associated with severe non-spherocytic haemolytic anaemia. Each variant shows a reduced K0.5 PEP (the value of the intercept of the abscissa on the Hill plot) and reduced Hill coefficient; FDP activation and ATP inhibition are less than normal and utilization of GDP is increased. The variants are slightly less inhibited by 2,3DPG than controls but require more FDP to relieve this inhibition. Cases 1 and 2 have decreased thermostability but case 3 is normal. The mutant enzymes are further distinguished by their affinity for FDP. Their kinetic and physicochemical properties are compared with other known cases with high affinity for PEP and discussed in terms of a R in equilibrium to T model model for allosteric enzymes.
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
August 1968,
Lancet (London, England),
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
January 1983,
Clinical and laboratory haematology,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
January 1975,
Acta biologica et medica Germanica,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
June 1982,
Biochimica et biophysica acta,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
July 2009,
Biological trace element research,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
July 1973,
Biochemistry,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
January 1974,
Enzyme,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
February 1982,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
December 1982,
Clinica chimica acta; international journal of clinical chemistry,
G E Elder, and
T R Lappin, and
B E Lawson, and
J M Bridges
January 1994,
Acta haematologica,
