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A simplified liquid chromatographic assay for epoxide hydrolase (E.C. 3.3.2.3) is described. The enzyme substrate employed, 3-(p-nitrophenoxy)-1,2-propene oxide, is commercially available and is readily resolved by reversed-phase liquid chromatography from the enzymatic hydration product, 3-(p-nitrophenoxy)-1,2-propane diol. As little as 100 pmol of the diol can be detected by absorbance monitoring at 315 nm. This assay has been employed to measure epoxide hydrolase in rat liver microsomes from animals treated with the dietary antioxidant, 2(3)-tert.-butyl-4-hydroxyanisole, a known inducer of the enzyme. The assay can also be used to measure rat liver cytosolic epoxide hydrolase.
K A Giuliano, and
E P Lau, and
R R Fall
June 1987,
Journal of chromatography,
K A Giuliano, and
E P Lau, and
R R Fall
August 1975,
Analytical biochemistry,
K A Giuliano, and
E P Lau, and
R R Fall
February 1980,
Analytical biochemistry,
K A Giuliano, and
E P Lau, and
R R Fall
October 2003,
Analytical biochemistry,
K A Giuliano, and
E P Lau, and
R R Fall
May 2004,
Biotechnology letters,
K A Giuliano, and
E P Lau, and
R R Fall
January 1975,
Annals of clinical biochemistry,
K A Giuliano, and
E P Lau, and
R R Fall
July 1985,
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology,
K A Giuliano, and
E P Lau, and
R R Fall
August 1980,
Analytical biochemistry,
K A Giuliano, and
E P Lau, and
R R Fall
December 2001,
Journal of biochemical and biophysical methods,
K A Giuliano, and
E P Lau, and
R R Fall
October 1998,
Journal of chromatographic science,
