A technique for the recovery of nematodes from ruminants by migration from gastro-intestinal ingesta gelled in agar: large-scale application. 1980

J A van Wyk, and H M Gerber, and H T Groeneveld

A gelled-agar technique for worm recovery was adapted to facilitate the recovery of larval and adult nematodes from the total ingesta of large numbers of sheep. The technique was also used to recover nematodes from 4 calves. In one trial involving 120 sheep, 100% of 2013 4th stage larvae (L4) and 92,1% of 134,205 adult Haemonchus contortus migrated from the agar preparations. Highly significantly more male than female worms failed to migrate. Using 1 x 1/10 aliquot to estimate the numbers of worms that failed to migrate from the agar, the mean error in the total worm count (worms that migrated plus those that failed to migrate) per sheep was 2,2%; with an examination of 2 x 1/10 aliquot the error was 1,7%. We concluded from this that the gelled-agar method may be of value for quantitative worm recovery, for example, in anthelmintic tests. In a second trial, 98,5% of 17,056 L4 and adult nematodes of 5 genera migrated from the ingesta of 4 calves and 96,4% of 62,597 L4 and adult nematodes of 9 species from the ingesta of 15 sheep. In general, L4 migrated slightly more efficiently than adult worms. In sheep and, to a lesser extent, in calves, Haemonchus spp. did not migrate as efficiently as the other genera such as Ostertagia, Trichostrongylus, Nematodirus, Oesophagostomum, Marshallagia and Chabertia.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008297 Male Males
D010270 Parasite Egg Count Determination of parasite eggs in feces. Count, Parasite Egg,Counts, Parasite Egg,Egg Count, Parasite,Egg Counts, Parasite,Parasite Egg Counts
D010274 Parasitology The study of parasites and PARASITIC DISEASES. Medical Parasitology,Parasitology, Medical
D011309 Preservation, Biological The process of protecting various samples of biological material. Biological Preservation,Preservation, Biologic,Biologic Preservation
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260 Female Females
D005615 Freezing Liquids transforming into solids by the removal of heat. Melting
D006189 Haemonchus A genus of parasitic nematode worms which infest the duodenum and stomach of domestic and wild herbivores, which ingest it with the grasses (POACEAE) they eat. Infestation of man is accidental. Hemonchus
D000018 Abomasum The fourth stomach of ruminating animals. It is also called the "true" stomach. It is an elongated pear-shaped sac lying on the floor of the abdomen, on the right-hand side, and roughly between the seventh and twelfth ribs. It leads to the beginning of the small intestine. (From Black's Veterinary Dictionary, 17th ed) Abomasums

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