-transparent.png){kind=logo}
The macronuclear rRNA genes of Tetrahymena thermophila contain an 0.4 kb intervening sequence in the 26S rRNA coding region. The sequence is represented within the primary transcription product. We demonstrate in this paper that the enzyme activities necessary for the endonucleolytic cleavage as well as for the ligation of the transcript are associated with the isolated. The intervening sequence is excised as an unique molecule, which is stable in vitro. About 50% of the in vitro synthesized RNA is processed. Faithful in vitro transcription occurs in the presence of the divalent ions Mg2+, Mn2+ and Co2+ while processing takes place only in the presence of Mg2+. The absolute requirement for Mg2+ in the excision reaction enables us to synthesize labelled pre-rRNA in the presence of Mn2+ or Co2+. The synthesized RNA can be used as a substrate in studies of th processing enzymes in vitro.
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
February 1980,
Cell,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
November 1975,
European journal of biochemistry,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
May 1986,
Journal of molecular biology,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
April 1972,
Biochimica et biophysica acta,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
December 1981,
Cell,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
January 1992,
Developmental genetics,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
April 1986,
Cell,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
November 1978,
Nucleic acids research,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
November 1979,
Proceedings of the National Academy of Sciences of the United States of America,
M Carin, and
B F Jensen, and
K D Jentsch, and
J C Leer, and
O F Nielsen, and
O Westergaard
February 1981,
Cell,
