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Quantitative estimation of free fatty acids was carried out in liver mitochondria of normal and thyroidectomized rabbits by means of two procedures--spectrophotometry using rhodamine 6Zh and a fluorescent method with 1,8-aniline naphthalene sulfonate /ANS/ as a dye. The use of ANS enabled to estimate quantitatively the free fatty acids beginning from 25 nmol in a sample /with eggor of about 17%/ by visual evaluation of the spot areas. As compared with the rhodamine procedure, use of ANS permitted to increase 5-fold the sensitivity of free fatty acid estimation, to elevate 6-fold the accuracy of the analysis, to overcome the step of fatty acid elution from chromatogramms and to decrease the time required for analysis. The data obtained suggest that the hyperthyroid state in rabbits was accompanied by an increase in the phospholipase A2 activity in liver mitochondria.
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
June 1975,
Archives of biochemistry and biophysics,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
May 2004,
Biochemical and biophysical research communications,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
March 1996,
Biochemistry and molecular biology international,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
March 1977,
Canadian journal of biochemistry,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
January 1991,
Methods in enzymology,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
October 1981,
European journal of biochemistry,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
June 1977,
Japanese journal of pharmacology,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
August 1995,
Analytical biochemistry,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
January 1972,
Biochimica et biophysica acta,
V K Fedorov, and
Z P Cheremisina, and
A I Deev, and
G L Neugodova, and
A I Marzoev
January 1979,
Ukrainskii biokhimicheskii zhurnal (1978),
