-transparent.png){kind=logo}
In this work we present a method which evaluates the facility of applying and reproducing ion fluxes in human cells using radioactive 22Na as a tracer. Intracellular sodium concentration, rate constants for total (oKNa TOT), ouabain-sensitive (oKNa OUABs) and ouabain-insensitive (oKNa OUABins), sodium efflux and relative effluxes obtained by multiplying the rate constant by the sodium concentration were measured in the red cells of 20 normal subjects. Our results have been shown to be comparable with those obtained in other reports and show a statistically significant relationship between intracellular sodium concentration and the rate constant for active sodium efflux: one would conclude that the intracellular sodium constant probably depends on the activity of the sodium pump genetically determined in each individual. Since such a method is precise can be exactly reproduced, it can be applied to the study of cellular metabolism of different clinical disorders characterized by significant fluid and electrolyte imbalances.
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
March 1993,
The Journal of membrane biology,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
January 1974,
Przeglad lekarski,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
March 1975,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
June 2001,
Biochimica et biophysica acta,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
July 1980,
Nature,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
June 1986,
European journal of biochemistry,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
January 1991,
Sheng li ke xue jin zhan [Progress in physiology],
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
January 1980,
La Medicina del lavoro,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
January 1976,
Rinsho byori. The Japanese journal of clinical pathology,
L Borghi, and
M Canali, and
A Curti, and
A Montanari, and
P Perinotto, and
A Novarini
November 1983,
The American journal of physiology,
