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A new procedure for microelectrophoretic delivery of horseradish peroxidase (HRP) is described. A dilute solution (1.5--3% w/v) of fluorescein isothiocyanate-conjugated HRP is deposited according to a standard microelectrophoretic method and the tissue reacted according to a sensitive histochemical procedure. A major advantage of this method is the reduced secondary diffusion of the enzyme present at the injection site.
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