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Gas-chromatographic analysis of the fatty acids (P/S ratio) in 212 samples of amniotic fluid, 35 samples of vernix caseosa and 35 samples of the pellets obtained after centrifugation of the amniotic fluid were carried out to evaluate the effects of contaminants that might be present in amniotic fluid on the P/S ratio. The P/S ratio is used as an index of the degree of maturity of the fetal or neonatal lungs. Analysis of variance applied to the means of the P/S values for each of above materials showed them to be significantly different and attests to the importance of centrifugation for obtaining valid results. We propose a standard procedure of centrifugation for 60 minutes at 3500 X g followed by extraction and gas-chromatography as a rapid, valid way to measure the P/S ratio.
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
January 1975,
Annali di ostetricia, ginecologia, medicina perinatale,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
October 1977,
Zeitschrift fur Geburtshilfe und Perinatologie,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
February 1979,
American journal of obstetrics and gynecology,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
January 1977,
Clinical chemistry,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
February 1975,
American journal of obstetrics and gynecology,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
March 1980,
Clinica chimica acta; international journal of clinical chemistry,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
January 1979,
Annales de biologie clinique,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
January 1974,
British medical journal,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
January 1982,
The Journal of reproductive medicine,
F Diani, and
P B Lantieri, and
S Ciangherotti, and
E Ferraresi
December 1979,
British journal of obstetrics and gynaecology,
