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[Characterization by RFLP of DNAs from Campylobacter jejuni Lior serotype reference strains and clinical isolates and detection of C. jejuni by DNA-probe]. 1995

Y Shibata, and J Yatsuyanagi, and S Saito, and H Saito, and M Morita, and K Amano
Central Research Laboratory, Akita University School of Medicine, Japan.

The Lior serotype reference strains and clinical isolates of Campylobacter jejuni were compared in the restriction fragment length polymorphism (RFLP) pattern to distinguish C. jejuni strains. These reference strains showed RFLP patterns different from one to another, while the patterns of some isolates were not coincident with those of the same serotype reference strains. Furthermore, we tried to hybridize HindIII-digested fragments from these strains with the DNA probe encoding the 46-kDa protein of C. jejuni by Southern and slot blottings. The 1.8-kbp fragments from all strains of C. jejuni hybridized with this probe, but those from other species of Campylobacter or enterobacteria did not. These results indicate that the Lior serotype is unrelated with the RFLP pattern of DNA of C. jejuni strains, but the DNA probe is useful to detect C. jejuni.

UI MeSH Term Description Entries
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D015342 DNA Probes Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal
D016123 Campylobacter jejuni A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals. Campylobacter fetus subsp. jejuni,Vibrio hepaticus,Vibrio jejuni

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