Biomaterial Database

Thymidine-suicide of human granulocytic progenitor cells (CFU-C). 1978

H P Lohrmann

The quantitative and qualitative changes of human granulopoietic stem cells (CFU-C) after perturbation give a comprehensive view of their reaction pattern. The proliferative state of CFU-C may be assessed using the 3H-Thymidine (3H-TdR) technique. This technique is well established in experimental animals. In order to utilize it for the study of human CFU-C, the kinetics and the variables affecting the suicide of human CFU-C were analysed in detail. From the results, it appears that under optimal conditions considerable higher 3H-TdR concentrations (greater than or equal to 100/muCi/ml) have to be utilized than have been reported to be satisfactory in the murine system. Colony growth inhibition is S-phase-specific; if reutilization of the label occurs in vitro, it does not detectably affect colony growth. Minimum exposure of CFU-C to 3H-TdR is 20 minutes. Using these optimal suicide conditions, human marrow CFU-C can be shown to be a highly proliferating cell population.

UI	MeSH Term	Description	Entries
D007399	Interphase	The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).	Interphases
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D003114	Colony-Forming Units Assay	A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.	Clonogenic Cell Assay,Stem Cell Assay,Clonogenic Cell Assays,Colony Forming Units Assays,Colony-Forming Units Assays,Stem Cell Assays,Assay, Clonogenic Cell,Assay, Colony-Forming Units,Assay, Stem Cell,Assays, Clonogenic Cell,Assays, Colony-Forming Units,Assays, Stem Cell,Colony Forming Units Assay
D006098	Granulocytes	Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.	Granulocyte
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013936	Thymidine	A nucleoside in which THYMINE is linked to DEOXYRIBOSE.	2'-Deoxythymidine,Deoxythymidine,2' Deoxythymidine
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In