BIOMAT Database Logo BIOMAT Database Logo

Innervation-independent changes in the mRNAs encoding tyrosine hydroxylase and the norepinephrine transporter in rat adrenal medulla after high-dose reserpine. 1995

J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Laboratory of Molecular Neurobiology, Burke Medical Research Institute, White Plains, NY 10605, USA.

To determine whether a trans-synaptic mechanism triggered the effects of reserpine on adrenomedullary mRNAs encoding the norepinephrine transporter and tyrosine hydroxylase, we administered 10 mg/kg reserpine to rats after unilateral splanchnicotomy, and examined their adrenal medullas using quantitative in situ hybridization. Splanchnicotomy did not alter the decrease in norepinephrine transporter mRNA that follows reserpine administration, but diminished the reserpine-induced increase in tyrosine hydroxylase mRNA by almost 80%. Despite the latter effect, reserpine still induced a significant increase in tyrosine hydroxylase mRNA in denervated adrenal medullas, compared to vehicle-treated adrenal medullas. These results show that a trans-synaptic mechanism does not trigger the decrease in adrenomedullary norepinephrine transporter mRNA following reserpine. In addition, an innervation-independent mechanism mediates a portion of the reserpine-induced increase in adrenomedullary tyrosine hydroxylase mRNA.

UI MeSH Term Description Entries
D008297 Male Males
D012110 Reserpine An alkaloid found in the roots of Rauwolfia serpentina and R. vomitoria. Reserpine inhibits the uptake of norepinephrine into storage vesicles resulting in depletion of catecholamines and serotonin from central and peripheral axon terminals. It has been used as an antihypertensive and an antipsychotic as well as a research tool, but its adverse effects limit its clinical use. Raunervil,Raupasil,Rausedil,Rausedyl,Serpasil,Serpivite,V-Serp,V Serp
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D000313 Adrenal Medulla The inner portion of the adrenal gland. Derived from ECTODERM, adrenal medulla consists mainly of CHROMAFFIN CELLS that produces and stores a number of NEUROTRANSMITTERS, mainly adrenaline (EPINEPHRINE) and NOREPINEPHRINE. The activity of the adrenal medulla is regulated by the SYMPATHETIC NERVOUS SYSTEM. Adrenal Medullas,Medulla, Adrenal,Medullas, Adrenal
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013153 Splanchnic Nerves The major nerves supplying sympathetic innervation to the abdomen. The greater, lesser, and lowest (or smallest) splanchnic nerves are formed by preganglionic fibers from the spinal cord which pass through the paravertebral ganglia and then to the celiac ganglia and plexuses. The lumbar splanchnic nerves carry fibers which pass through the lumbar paravertebral ganglia to the mesenteric and hypogastric ganglia. Nerve, Splanchnic,Nerves, Splanchnic,Splanchnic Nerve
D014446 Tyrosine 3-Monooxygenase An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 1.14.16.2. Tyrosine Hydroxylase,3-Monooxygenase, Tyrosine,Hydroxylase, Tyrosine,Tyrosine 3 Monooxygenase
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D017403 In Situ Hybridization A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. Hybridization in Situ,Hybridization, In Situ,Hybridizations, In Situ,In Situ Hybridizations

Related Publications

J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Water deprivation and rat adrenal mRNAs for tyrosine hydroxylase and the norepinephrine transporter.
June 1997, The American journal of physiology,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Multiple complexes involved in tyrosine hydroxylase mRNA stability in rat adrenal medulla, after reserpine stimulation.
January 2001, Molecular and cellular neurosciences,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Regulation of norepinephrine transporter and tyrosine hydroxylase mRNAs after kainic acid-induced seizures.
September 1999, Brain research,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Biosynthesis of tyrosine hydroxylase in rat adrenal medulla after exposure to cold.
November 1974, Proceedings of the National Academy of Sciences of the United States of America,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Prenatal reserpine administration: permanent changes in adrenal tyrosine hydroxylase and dopamine beta-hydroxylase.
January 1975, Psychopharmacology communications,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Histochemical and volumetric changes in the adrenal medulla of rat and mouse after reserpine administration.
January 1971, The Journal of the Egyptian Medical Association,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Effect of dopamine on tyrosine hydroxylase in cultured rat adrenal medulla.
April 1988, Biochemical pharmacology,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Tyrosine hydroxylase expression in rat adrenal medulla: influence of age and cold.
August 1995, Pharmacology, biochemistry, and behavior,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Cycloheximide increases proenkephalin and tyrosine hydroxylase gene expression in rat adrenal medulla.
June 2000, Molecular pharmacology,
J F Cubells, and H Baker, and B T Volpe, and G P Smith, and S S Das, and T H Joh
Regulation of tyrosine hydroxylase activity by muscarinic agonists in rat adrenal medulla.
August 1998, The Journal of pharmacology and experimental therapeutics,
Copied contents to your clipboard!