-transparent.png){kind=logo}
Fractionation studies were performed of an immune complex-like material measured in rheumatoid synovial fluid using a biological assay based on histamine release from guinea pig lung. This material was eluted from Sepharose 6B in the excluded fraction, from DEAE cellulose predominantly in the third buffer, and from polyacrylamide electrophoresis in a cathodal position. Heterogeneity of the complexes was evident on iso-electric focussing, with one peak of activity at a pH of 4.2--5.6 and the other at pH 6.8--8.6. Purification of the material was limited by a loss of activity which occurred on using three fractionation procedure in sequence. This instability was avoided in the case of Sepharose 6B by adding 0.1% bovine or rabbit albumin to the elution buffer.
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
July 1968,
Clinical and experimental immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
July 1968,
Clinical and experimental immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
June 1974,
The Journal of rheumatology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
July 1968,
Clinical and experimental immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
July 1969,
Clinical and experimental immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
June 1985,
Journal of clinical & laboratory immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
May 1974,
Clinical and experimental immunology,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
January 1973,
Arthritis and rheumatism,
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
August 1963,
Journal of immunology (Baltimore, Md. : 1950),
R Yue, and
E Tackaberry, and
D McAvoy, and
C Shustik, and
I Broder
August 1977,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
