-transparent.png){kind=logo}
There is no available monoclonal antibody which reacts specifically recognizes human tyrosinase. Employing a synthetic peptide, MEKEDYHSLYQSHL, corresponding to the carboxyl terminus of human tyrosinase as an immunogen, we produced a mouse monoclonal antibody MAT-1 of the IgG1 isotype. The epitope for MAT-1 was determined to be EDYH, the sequence of which is not present in human tyrosinase-related protein-1 (TRP-1) or tyrosinase-related protein-2 (TRP-2). By transient expression assays and immunofluorescence technique, we show that MAT-1 reacts specifically with cells expressing human tyrosinase cDNA but not with cells expressing TRP-1 or TRP-2 cDNA. The results of immunohistochemical staining also confirmed that MAT-1 reacts specifically with epidermal melanocytes in human skin sections. MAT-1 should be invaluable for studying the interaction between tyrosinase and TRPs and for detecting the changes in the levels of tyrosinase expression. In addition, MAT-1 should be useful as a sensitive immunohistochemical tool for investigation of various pigmentary disorders and possibly for the diagnosis of melanoma.
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
June 1996,
Journal of neuropathology and experimental neurology,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
July 1989,
Laboratory investigation; a journal of technical methods and pathology,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
May 2012,
The Journal of biological chemistry,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
August 1990,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
February 2020,
Monoclonal antibodies in immunodiagnosis and immunotherapy,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
December 2009,
Biochemical and biophysical research communications,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
July 1983,
The Journal of biological chemistry,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
July 1987,
Clinical chemistry,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
March 2013,
Biochemical and biophysical research communications,
H Takimoto, and
S Suzuki, and
S Masui, and
K Shibata, and
Y Tomita, and
S Shibahara, and
H Nakano
January 1996,
Acta neuropathologica,
