-transparent.png){kind=logo}
The inhibitory effect of antisense phosphorothioate oligodeoxyribonucleotides (S-oligos) on the production of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) was studied using Hep G2 cells transfected with hepatitis B virus (HBV). Synthetic antisense oligos (15-mers) directed against the cap site of mRNA transcribed from the SP II promoter and regions of the translational initiation site of the S gene showed a sequence-specific, dose-dependent inhibitory effect on HBV gene expression between concentrations of 1.0 microM-5.0 microM. Oligos directed against the middle of the S gene had little effect on HBsAg and HBeAg expression, as did non-complementary random-sequence control. The cells remained viable throughout the experiments and no morphological abnormalities were observed with antisense S-oligos at concentrations below 20.0 microM. These results suggest a therapeutic potential for antisense oligonucleotides in the treatment of patients who are chronically infected with HBV.
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
September 1995,
Hepatology (Baltimore, Md.),
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
March 1993,
The EMBO journal,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
August 2002,
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
January 1996,
Biochemical and biophysical research communications,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
May 1991,
Lancet (London, England),
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
March 2003,
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
December 1995,
Journal of biochemistry,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
January 1993,
Folia biologica,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
June 1992,
The Journal of biological chemistry,
Z Q Yao, and
Y X Zhou, and
A L Wang, and
X F Bai, and
W S Yang
February 1999,
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics,
