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A radiocompetitive estimation of 25-hydroxyvitamin D in human serum is described. After extraction, a stage of purification by chromatography on a Sephadex LH 20 column permits separation of 25-hydroxyvitamin D from Vitamin D and other metabolites. The reaction of radiocompetition uses as tracer tritiated 25-hydroxycholecalciferol and as source of binding protein, rat renal cytosol. The characteristics of the binding protein and the presence in human plasma of significant quantities of 24,25-dihydroxyvitamin D render necessary the chromatographic stage. This technic permits one to measure simply and specifically 25-hydroxyvitamin D in the human serum, of which normal levels (average +/- standard deviation) are 12.88 +/- 5.42 microgram/l 3.2.10(-8) + 1.35.10(-8) mol/l).
S Guillemant, and
R Kremer
December 1981,
Deutsche medizinische Wochenschrift (1946),
S Guillemant, and
R Kremer
February 1977,
Polskie Archiwum Medycyny Wewnetrznej,
S Guillemant, and
R Kremer
March 1978,
Nihon Jinzo Gakkai shi,
S Guillemant, and
R Kremer
September 1976,
Casopis lekaru ceskych,
S Guillemant, and
R Kremer
February 1981,
Annals of clinical research,
S Guillemant, and
R Kremer
January 2011,
Endokrynologia Polska,
S Guillemant, and
R Kremer
December 2017,
Diabetes research and clinical practice,
S Guillemant, and
R Kremer
January 1983,
Voprosy meditsinskoi khimii,
S Guillemant, and
R Kremer
December 1984,
The Veterinary record,
S Guillemant, and
R Kremer
April 1978,
Archives of disease in childhood,
