Biomaterial Database

Cell-wall proteins of Staphylococcus aureus : a kinetic study of release by lysostaphin. 1978

B Poutrel, and G Dubray

The kinetics of cell-wall hydrolysis of Staphylococcus aureus (strain Cowan I) by lysostaphin was studied by turbidity, quantitative determination of hexosamines, phosphorus, total proteins, protein A and by electron microscopy. In our experimental conditions, the lytic activity of lysostaphin was very fast and no characteristic cell-wall structures could be seen after five minutes. The release of hexosamines, phosphorus and protein A was synchronous and could be recorded even though turbidity had become stable. At the end of the hydrolysis, the totality of these components was solubilized. Total proteins represented about 14% of the cell-wall dry weight. During hydrolysis protein A was the only protein solubilized.

UI	MeSH Term	Description	Entries
D008248	Lysostaphin	A 25-kDa peptidase produced by Staphylococcus simulans which cleaves a glycine-glcyine bond unique to an inter-peptide cross-bridge of the STAPHYLOCOCCUS AUREUS cell wall.
D010758	Phosphorus	A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.	Black Phosphorus,Phosphorus-31,Red Phosphorus,White Phosphorus,Yellow Phosphorus,Phosphorus 31,Phosphorus, Black,Phosphorus, Red,Phosphorus, White,Phosphorus, Yellow
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D006595	Hexosamines	AMINO SUGARS created by adding an amine group to a hexose sugar.	Hexosamine
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001433	Bacteriolysis	Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.	Bacteriolyses
D013205	Staphylococcal Protein A	A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.	Protein A,Protein A, Staphylococcal
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.