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The hypothesis that P-glycoprotein (P-gp) mediates the renal secretion of organic cations was tested by functional expression of mRNAs in the Xenopus laevis oocyte system. Efflux of 2'-deoxytubercidin (dTub), a substrate for the renal organic cation transporter (OCT) but not for P-gp, was enhanced by injection of renal mRNA but not by injection of mRNA from P-gp-overexpressing cells (MDCK cells transduced with the cDNA for human MDR1). The functional capacity of the MDCK-MDR mRNA was established by its ability to reduce the steady-state uptake of a classical P-gp substrate, vinblastine. Thus, these data indicate OCT and P-gp to be distinct entities. The Xenopus oocyte system provides a functional approach to further characterize the OCT.
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
April 1992,
The Biochemical journal,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
September 1992,
Molecular and cellular biochemistry,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
June 1997,
Journal of pharmaceutical sciences,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
July 1994,
The American journal of physiology,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
March 1991,
The Journal of biological chemistry,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
August 2003,
Biochemical and biophysical research communications,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
March 1991,
Naunyn-Schmiedeberg's archives of pharmacology,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
August 1994,
Biochemical pharmacology,
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
January 2007,
Methods in molecular biology (Clifton, N.J.),
J A Nelson, and
A Dutt, and
L H Allen, and
D A Wright
May 1997,
Pediatric research,
