Biomaterial Database

Production of interferon in human fibroblast cultures: effect of temperature. 1978

A Sypuła, and J Zielińska-Jenczylik

To obtain several harvests of interferon from the same culture the human fibroblasts have been incubated at various temperatures after the induction with virus. Preincubation of the culture infected with Newcastle Disease virus (NDV) at 37 degrees C for 8-10 hrs before the transfer of it to 25 degrees C resulted in the optimal harvest of interferon at 24th and 48th hrs after the induction. Also the preincubation of the culture infected with NDV for 8-10 hrs at 25 degrees C before its transfer to 37 degrees C resulted in better harvests of interferon than that obtained after the constant incubation at 37 degrees C. The same preparation of NDV could be used repeatedly 10 times without substantial loss of its interferon-inducing activity.

UI	MeSH Term	Description	Entries
D007372	Interferons	Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.	Interferon
D009522	Newcastle disease virus	The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food.	Avian Paramyxovirus 1,Paramyxovirus 1, Avian
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor