BIOMAT Database Logo BIOMAT Database Logo

Hyperthermic induction of premature chromosome condensation in human lymphocytes. 1995

K Prabhakara, and S K Murthy
Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India.

Hyperthermic induction of chromosomal aberrations was examined in human lymphocytes. For this purpose, whole blood cultures were exposed to an elevated temperature (43 degrees C) at the 46th h of culture initiation, followed by 24 h of recovery. Detailed chromosomal analysis showed significantly higher levels of aberrations as compared to control cultures of the same individuals. These aberrations include breaks, gaps of both chromosome and chromatid type, aneuploidy and high levels of polyploidy. The interesting observation was the occurrence of S-phase premature chromosome condensation (PCC). The S-phase PCC observed here could be due to asynchronous nuclei induced by hyperthermia. A possible interpretation for the occurrence of asynchronous nuclei leading to the induction of S-phase PCC is discussed.

UI MeSH Term Description Entries
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008938 Mitosis A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002842 Chromatids Either of the two longitudinally adjacent threads formed when a eukaryotic chromosome replicates prior to mitosis. The chromatids are held together at the centromere. Sister chromatids are derived from the same chromosome. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Chromatid
D002869 Chromosome Aberrations Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS. Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D002877 Chromosomes, Human Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual. Chromosome, Human,Human Chromosome,Human Chromosomes
D005260 Female Females
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016196 S Phase Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome. S Period,Period, S,Periods, S,Phase, S,Phases, S,S Periods,S Phases

Related Publications

K Prabhakara, and S K Murthy
[Premature chromosome condensation. I. Comparison of various methods of premature chromosome condensation induction].
July 1983, Zhonghua zhong liu za zhi [Chinese journal of oncology],
K Prabhakara, and S K Murthy
[Induction of interphase chromosome condensation in cultured human lymphocytes].
August 1975, Tsitologiia,
K Prabhakara, and S K Murthy
Premature chromosome condensation in human leukemia.
April 1976, Blood,
K Prabhakara, and S K Murthy
Nucleolus organizer regions in human lymphocytes as studied with premature chromosome condensation.
February 1990, Human genetics,
K Prabhakara, and S K Murthy
An alternative approach for the induction of premature chromosome condensation in human peripheral blood lymphocytes using mitotic Akodon cells.
February 2020, International journal of radiation biology,
K Prabhakara, and S K Murthy
Premature chromosome condensation in human cervical carcinoma.
February 1987, Cancer genetics and cytogenetics,
K Prabhakara, and S K Murthy
Chromosome aberrations induced in human lymphocytes by 3.45 MeV alpha particles analyzed by premature chromosome condensation.
October 1999, Radiation research,
K Prabhakara, and S K Murthy
Induction of premature chromosome condensation in Drosophila melanogaster-rat heterokaryons.
January 1987, Chromosoma,
K Prabhakara, and S K Murthy
Premature chromosome condensation in human large bowel cancer.
April 1981, Cancer genetics and cytogenetics,
K Prabhakara, and S K Murthy
Mammalian cell fusion: induction of premature chromosome condensation in interphase nuclei.
May 1970, Nature,
Copied contents to your clipboard!