Biomaterial Database

A radioimmunoassay for 3,5,3'-triiodothyronine in chicken serum. 1978

J D May

A double-antibody radioimmunoassay is described for 3,5,3'-triiodothyronine (T3) in chicken serum. Primary antibody produced in a rabbit had negligible crossreactivity with 3,3,5'-triiodothyronine and thyroxine. The primary antibody diluted 1:1000 bound 50% of a tracer dose of 125I-T3, and a standard curve of 0 to 800 pg/tube was used. Percent binding ranged from 14.1 to 54.1 for a standard curve. Serum volume ranging from 50 to 150 microliter tube did not affect assay results. Coefficient of variation was 2.8% within the assay and 5.5% between assays. The inhibitor 8-anilino-1-naphthalene sulfonic acid was necessary for recovery of T3 from spiked samples. Young chickens had reduced serum T3 concentrations when fed diets containing .1% thiouracil for 14 days when compared with serum T3 concentrations of chickens fed the diet without thiouracil. Feeding .2% thiouracil for 3 days did not affect serum T3 concentration.

UI	MeSH Term	Description	Entries
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D013889	Thiouracil	Occurs in seeds of Brassica and Crucifera species. Thiouracil has been used as antithyroid, coronary vasodilator, and in congestive heart failure although its use has been largely supplanted by other drugs. It is known to cause blood dyscrasias and suspected of terato- and carcinogenesis.
D014284	Triiodothyronine	A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.	Liothyronine,T3 Thyroid Hormone,3,3',5-Triiodothyronine,Cytomel,Liothyronine Sodium,Thyroid Hormone, T3