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A new dyed substrate was prepared for the rapid determination of endo-1,4-beta-glucanases. Carboxymethylcellulose was coupled with Ruthenium red to obtain a violet powder, which was stable enough to allow for reproducible assays. The spontaneous reaction is based on ionic interactions between the negatively charged carboxymethylcellulose and the complex cation of the dye. The enzymic assay is based on spectrophotometric measurement at 535 nm of the enzyme-released dyed fragments with low-molecular-weight filterable through a 0.22 microns filter. The release of coloured fragments from this dyed substrate was proportional to its solubilization. The absorbance was directly proportional to the enzyme amount in the range 0.5-5.5 mU enzyme (A535 = 0.1-0.95). This enzymic assay is advantageous for both rapid time of analysis and sensitivity.
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
January 1985,
Analytical biochemistry,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
January 1985,
Analytical biochemistry,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
March 1978,
Biochimica et biophysica acta,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
December 1988,
Gene,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
December 1991,
Biotechnology and applied biochemistry,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
March 1978,
Biochimica et biophysica acta,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
August 2003,
Analytical biochemistry,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
July 1984,
Plant physiology,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
September 2003,
Journal of experimental botany,
A Rescigno, and
A C Rinaldi, and
N Curreli, and
A Olianas, and
E Sanjust
October 2005,
Acta biochimica et biophysica Sinica,
