Biomaterial Database

Changes in cellular concentration of DNA-dependent RNA polymerases A and B during regeneration of rat liver. 1978

Z Krawczyk, and M Chorazy

1. DNA-dependent RNA polymerases A and B were solubilized from rat liver nuclei at different intervals after partial hepatectomy, and chromatographed on DEAE-Sephadex A-25. Activity of the solubilized RNA polymerases remained unchanged till 6 h after hepatectomy, then started to increase reaching a maximum (350% and 150% of control for the A and B enzyme, respectively) at the 18th hour of regeneration, and was still high at the 36th hour of regeneration. 2. RNA polymerases A and B were extracted and extensively purified from the nuclei of normal and regenerating rat liver. No marked differences in the specific activities between the analogous purified enzymes from normal and regenerating liver were observed, thus the increase in RNA polymerase activities (especially marked in the case of enzyme A) observed after partial hepatectomy is probably due to a real increase in the quantities of enzymes. 3. Concentration of RNA polymerase A in hepatocyte increases from 1.3 x 10(4) (normal liver) to 7.5 x 10(4) (18 h after hepatectomy) molecules per haploid genome. The concentration of polymerase B increases from 3.4 x 10(4) to 5.5 x 10(4) molecules per haploid genome, respectively.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008115	Liver Regeneration	Repair or renewal of hepatic tissue.	Liver Regenerations,Regeneration, Liver,Regenerations, Liver
D008297	Male		Males
D002845	Chromatography	Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.	Chromatographies
D000645	Ammonium Sulfate	Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.	Sulfate, Ammonium
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012318	RNA Polymerase I	A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. The enzyme functions in the nucleolar structure and transcribes DNA into RNA. It has different requirements for cations and salts than RNA polymerase II and III and is not inhibited by alpha-amanitin.	DNA-Dependent RNA Polymerase I,RNA Polymerase A,DNA Dependent RNA Polymerase I,Polymerase A, RNA,Polymerase I, RNA
D012319	RNA Polymerase II	A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.	DNA-Dependent RNA Polymerase II,RNA Pol II,RNA Polymerase B,DNA Dependent RNA Polymerase II
D012321	DNA-Directed RNA Polymerases	Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).	DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor