BIOMAT Database Logo BIOMAT Database Logo

Human monoclonal anti-D secreting heterohybridomas from peripheral B lymphocytes expanded in the CD40 system. 1994

J M Thompson, and J Lowe, and D F McDonald
Research and Development Laboratory, West Midlands Blood Transfusion Service, Edgbaston, Birmingham, UK.

Peripheral human B lymphocytes isolated from four immunised anti-D donors have been cultured in the CD40 system (Banchereau et al., 1991), prior to fusion to murine X63Ag8.653 plasmacytoma cells. High fusion efficiency was noted in all cases and immunoglobulin (IgG and IgM) was detected in nearly all heterohybrid containing wells. Only fusions using boosted donor lymphocytes generated specific anti-D secreting hybrids. A short period of culture of committed B cell precursors in the CD40 system before fusion appears to be both adequate and efficient in permitting generation of specific antibody-secreting hybrids.

UI MeSH Term Description Entries
D007518 Isoantibodies Antibodies from an individual that react with ISOANTIGENS of another individual of the same species. Alloantibodies
D002459 Cell Fusion Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization. Cell Fusions,Fusion, Cell,Fusions, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000944 Antigens, Differentiation, B-Lymphocyte Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin. Antigens, Differentiation, B-Cell,B-Cell Differentiation Antigens,B-Lymphocyte Differentiation Antigens,Blast-2 Antigen, B-Cell,Differentiation Antigens, B-Cell,Differentiation Antigens, B-Lymphocyte,Leu Antigens, B-Lymphocyte,Plasma Cell Antigens PC-1,Antigens, Differentiation, B Lymphocyte,Antigens, Plasma Cell, PC-1,B-Cell Blast-2 Antigen,Antigen, B-Cell Blast-2,Antigens, B-Cell Differentiation,Antigens, B-Lymphocyte Differentiation,Antigens, B-Lymphocyte Leu,B Cell Blast 2 Antigen,B Cell Differentiation Antigens,B Lymphocyte Differentiation Antigens,B-Lymphocyte Leu Antigens,Blast 2 Antigen, B Cell,Differentiation Antigens, B Cell,Differentiation Antigens, B Lymphocyte,Leu Antigens, B Lymphocyte,Plasma Cell Antigens PC 1
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D012204 Rh-Hr Blood-Group System Erythrocyte isoantigens of the Rh (Rhesus) blood group system, the most complex of all human blood groups. The major antigen Rh or D is the most common cause of erythroblastosis fetalis. Rh Factors,Rhesus Blood-Group System,Antigen D, Rh Blood Group,Blood-Group System, Rh-Hr,Blood-Group System, Rhesus,Blood-Group Systems, Rh-Hr,Blood-Group Systems, Rhesus,Factor, Rh,Factors, Rh,Rh Factor,Rh Hr Blood Group System,Rh-Hr Blood-Group Systems,Rhesus Blood Group System,Rhesus Blood-Group Systems,System, Rh-Hr Blood-Group,System, Rhesus Blood-Group,Systems, Rh-Hr Blood-Group,Systems, Rhesus Blood-Group
D015703 Antigens, CD Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation. CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation

Related Publications

J M Thompson, and J Lowe, and D F McDonald
Efficient preparation of human monoclonal antibody-secreting heterohybridomas using peripheral B lymphocytes cultured in the CD40 system.
February 1993, Journal of immunological methods,
J M Thompson, and J Lowe, and D F McDonald
Heterohybridomas secreting human monoclonal antibodies against Haemophilus influenzae type b.
April 1988, European journal of immunology,
J M Thompson, and J Lowe, and D F McDonald
Growing human B lymphocytes in the CD40 system.
October 1991, Nature,
J M Thompson, and J Lowe, and D F McDonald
Production of heterohybridomas secreting autoreactive and polyreactive human monoclonal antibodies.
January 1992, Scandinavian journal of immunology,
J M Thompson, and J Lowe, and D F McDonald
Requirements for the establishment of heterohybridomas secreting monoclonal human antibody to rhesus (D) blood group antigen.
November 1987, Journal of immunological methods,
J M Thompson, and J Lowe, and D F McDonald
Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual.
January 1982, The Journal of experimental medicine,
J M Thompson, and J Lowe, and D F McDonald
Human monoclonal anti-nuclear antibodies produced by human-mouse heterohybridomas.
January 1986, Clinical and experimental immunology,
J M Thompson, and J Lowe, and D F McDonald
Synergistic stimulation of human B lymphocytes by anti-CD40 monoclonal antibodies and synthetic lipopeptide analogues from Escherichia coli lipoprotein.
February 1994, Immunology,
J M Thompson, and J Lowe, and D F McDonald
Generation and characterization of secreting and nonsecreting human x mouse heterohybridomas obtained by in vitro immunization of human peripheral blood lymphocytes.
April 1991, Human antibodies and hybridomas,
J M Thompson, and J Lowe, and D F McDonald
Production of human monoclonal IgG and IgM antibodies with anti-D (rhesus) specificity using heterohybridomas.
May 1986, Immunology,
Copied contents to your clipboard!