Biomaterial Database

Alterations in the glomerular charge barrier in human lupus nephritis. 1994

M D al-Nawab, and D R Davies

Department of Histopathology, UMDS of Guy's Hospital, London, U.K.

Renal tissue taken from ten patients with proteinuria and diagnosed as suffering from human lupus nephritis was examined by a post-embedding charge labelling method using cationic gold (CG). The method allows localization of the anionic charge in the glomerular epithelial cell coat and/or glomerular basement membrane (GBM). Our results demonstrate that in human lupus nephritis there are gaps in the charge barrier associated with immune deposits found in the lamina rara externa of the GBM. These data suggest that the loss of glomerular fixed anionic charges in the GBM may play an important role in the development of proteinuria in patients suffering from lupus nephritis.

UI	MeSH Term	Description	Entries
D007678	Kidney Glomerulus	A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.	Glomerulus, Kidney
D008181	Lupus Nephritis	Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).	Glomerulonephritis, Lupus,Lupus Glomerulonephritis,Nephritis, Lupus,Glomerulonephritides, Lupus,Lupus Glomerulonephritides,Lupus Nephritides,Nephritides, Lupus
D011107	Polylysine	A peptide which is a homopolymer of lysine.	Epsilon-Polylysine,Poly-(Alpha-L-Lysine),Epsilon Polylysine
D011507	Proteinuria	The presence of proteins in the urine, an indicator of KIDNEY DISEASES.	Proteinurias
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000838	Anions	Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis.	Anion
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings