Biomaterial Database

Histochemical and biochemical studies of the kinetics of non-specific acid phosphatase in rat kidney. 1994

P J de Permentier, and F W Rost

School of Anatomy, University of New South Wales, Sydney, Australia.

Kinetic characteristics of non-specific acid phosphatase (orthophosphoric monoester phosphohydrolase, E.C.3.1.3.2.) from rat kidney were determined fluorometrically using 4-methylumbelliferyl phosphate as the substrate. Kinetic characteristics measured by similar methods both histochemically in cryostat sections and biochemically in tissue extracts were compared. Histochemical and biochemical methods gave essentially similar results in respect of Michaelis-Menten constants (Km), pH optima, effect of fluoride inhibition and the effect of changes in incubation temperatures in the range 10 degrees C to 37 degrees C. This confirms the validity of both methods, and also gives greater confidence that the enzyme in vitro closely approximates the properties of the enzyme as it functions in vivo.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D002384	Catalysis	The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.	Catalyses
D005260	Female		Females
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000135	Acid Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.	Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity