Molecular fingerprinting of Pasteurella multocida associated with progressive atrophic rhinitis in swine herds. 1994

I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616.

Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with gamma-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D (n = 51), nontoxigenic type D (n = 28), nontoxigenic type A (n = 16), and toxigenic type A (n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.

UI MeSH Term Description Entries
D010326 Pasteurella Infections Infections with bacteria of the genus PASTEURELLA. Pasteurellosis,Infections, Pasteurella,Infection, Pasteurella,Pasteurella Infection,Pasteurelloses
D004196 Disease Outbreaks Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS. Outbreaks,Infectious Disease Outbreaks,Disease Outbreak,Disease Outbreak, Infectious,Disease Outbreaks, Infectious,Infectious Disease Outbreak,Outbreak, Disease,Outbreak, Infectious Disease,Outbreaks, Disease,Outbreaks, Infectious Disease
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001315 Australia The smallest continent and an independent country, comprising six states and two territories. Its capital is Canberra. Canton and Enderbury Islands,Christmas Island,Christmas Island (Australia)
D012222 Rhinitis, Atrophic A chronic inflammation in which the NASAL MUCOSA gradually changes from a functional to a non-functional lining without mucociliary clearance. It is often accompanied by degradation of the bony TURBINATES, and the foul-smelling mucus which forms a greenish crust (ozena). Ozena,Atrophic Rhinitides,Atrophic Rhinitis,Ozenas,Rhinitides, Atrophic
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013553 Swine Diseases Diseases of domestic swine and of the wild boar of the genus Sus. Disease, Swine,Diseases, Swine,Swine Disease

Related Publications

I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
September 1954, Journal of the American Veterinary Medical Association,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
May 2009, Vaccine,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
November 1991, Australian veterinary journal,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
May 1990, DTW. Deutsche tierarztliche Wochenschrift,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
August 1991, DTW. Deutsche tierarztliche Wochenschrift,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
April 1991, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
January 1988, The Veterinary record,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
July 1989, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
January 1974, National Institute of Animal Health quarterly,
I A Gardner, and R Kasten, and G J Eamens, and K P Snipes, and R J Anderson
October 1992, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Copied contents to your clipboard!