BIOMAT Database Logo BIOMAT Database Logo

Cell-cycle analysis using continuous bromodeoxyuridine labeling and Hoechst 33358-ethidium bromide bivariate flow cytometry. 1994

M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Department of Human Genetics, University of Würzburg, Germany.

UI MeSH Term Description Entries
D001973 Bromodeoxyuridine A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors. BUdR,BrdU,Bromouracil Deoxyriboside,Broxuridine,5-Bromo-2'-deoxyuridine,5-Bromodeoxyuridine,NSC-38297,5 Bromo 2' deoxyuridine,5 Bromodeoxyuridine,Deoxyriboside, Bromouracil
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004996 Ethidium A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium Bromide,Homidium Bromide,Novidium,Bromide, Ethidium,Bromide, Homidium
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006690 Bisbenzimidazole A benzimidazole antifilarial agent; it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication; it also interferes with mitosis. Bisbenzimide,4-(5-(4-Methyl-1-piperazinyl)(2,5'-bi-1H-benzimidazol)-2'-yl)phenol, trihydrochloride,Bisbenzimidazole Trihydrochloride,Hoe-33258,Hoechst 33258,NSC-322921,Pibenzimol,Hoe 33258,Hoe33258,NSC 322921,NSC322921
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013194 Staining and Labeling The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts. Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings

Related Publications

M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method.
January 1997, Methods in molecular biology (Clifton, N.J.),
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Cell cycle analysis of synchronized Chinese hamster cells using bromodeoxyuridine labeling and flow cytometry.
January 1995, In vitro cellular & developmental biology. Animal,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Cell cycle analysis by combining the 5-bromodeoxyuridine/33258 Hoechst technique with DNA-specific ethidium bromide staining.
July 1981, Cytometry,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Cell cycle analysis of asynchronous cell populations by flow cytometry using bromodeoxyuridine label and Hoechst-propidium iodide stain.
January 1992, Cytometry,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Application of a bromodeoxyuridine-Hoechst/ethidium bromide technique for the analysis of radiation-induced cell cycle delays in asynchronous cell populations.
February 1996, International journal of radiation biology,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Flow cytometric cell cycle analysis using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation.
January 1979, Cell and tissue kinetics,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Bivariate flow cytometry DNA/BrdUrd analysis of plant cell cycle.
January 1999, Methods in cell science : an official journal of the Society for In Vitro Biology,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
In vitro bromodeoxyuridine labeling of malignant neoplasms. A comparative study with flow cytometry cell-cycle analysis.
June 1994, American journal of clinical pathology,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Cell cycle analysis using numerical simulation of bivariate DNA/bromodeoxyuridine distributions.
November 1985, Cytometry,
M Poot, and H Hoehn, and M Kubbies, and A Grossmann, and Y Chen, and P S Rabinovitch
Cell cycle analysis using flow cytometry.
February 1986, International journal of radiation biology and related studies in physics, chemistry, and medicine,
Copied contents to your clipboard!