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It has been shown that the gene ERG in 21q22 is rearranged in the t(16;21)(p11;q22) associated with acute myeloid leukemia (AML). ERG is a member of the ETS gene family and is fused with EWS in a subset of Ewing's sarcomas. EWS in 22q12 has a very high homology with FUS (also called TLS) in 16p11; the latter gene is rearranged in the t(12;16)(q13;p11) that characterizes myxoid liposarcoma. To investigate whether FUS is involved in the t(16;21) of AML, we used the Southern blot technique and polymerase chain reaction (PCR) to examine the bone marrow of a 3-year-old boy with a t(16;21)(p11;q22)-positive AML. Hybridization of Southern blot filters containing digested DNA with probes for FUS and ERG showed both germline and aberrant fragments. Using specific primers for the 5' part of FUS and the 3' part of ERG, we amplified a 4.4 kb genomic FUS/ERG DNA fragment from the leukemic sample. In a second PCR experiment, in which we used primers upstream of the 5' part of ERG and downstream of the 3' part of FUS, a 5.6 kb fragment was amplified. Blotting and hybridization with specific probes for FUS and ERG revealed that the amplified fragments consisted of FUS/ERG and ERG/FUS hybrid DNA. Both PCR fragments, when used as probes, detected germline ERG and FUS as well as aberrant fragments on Southern blot filters. The results suggest that the t(16;21) in AML leads to rearrangement and fusion of the FUS and ERG genes.(ABSTRACT TRUNCATED AT 250 WORDS)
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
December 2017,
Clinical case reports,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
October 2009,
The Korean journal of laboratory medicine,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
December 2005,
Leukemia & lymphoma,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
April 2024,
Blood science (Baltimore, Md.),
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
May 2023,
Zhonghua er ke za zhi = Chinese journal of pediatrics,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
January 2016,
Journal of the Association of Genetic Technologists,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
August 1997,
Blood,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
May 2015,
Oncology letters,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
June 2006,
Cancer genetics and cytogenetics,
I Panagopoulos, and
P Aman, and
T Fioretos, and
M Höglund, and
B Johansson, and
N Mandahl, and
S Heim, and
M Behrendtz, and
F Mitelman
September 2011,
Asia-Pacific journal of clinical oncology,
