-transparent.png){kind=logo}
Antisera were raised in rabbits against three peptides, representing amino acid sequences 150 to 159, 165 to 174, and 213 to 226 of mouse prion (PrP), which were synthesized by using a multiple antigenic peptide (MAP) system. The reactivities of these sera to PrP were examined by an enzyme-linked immunosorbent assay (ELISA), Western immunoblotting (WB), and immunohistochemical procedures. The results of both ELISA and WB showed that antisera to peptide sequence 150 to 159 (Ab150-159) did not react with purified mouse PrP. On the other hand, sera to the sequence 165 to 174 (Ab165-174) reacted weakly with purified mouse PrP, as detected by WB but not by ELISA. However, antiserum to peptide sequence 213 to 226 (Ab213-226) reacted strongly with mouse, Syrian hamster, and sheep PrP by WB and with mouse PrP as shown by the results of ELISA. Moreover, Ab213-226 clearly detected PrP immunohistochemically in mouse, Syrian hamster, and sheep brains affected with scrapie as well as in the brain of a cow with bovine spongiform encephalopathy. From these data, we conclude that rabbit antiserum against the MAP representing amino acid sequence 213 to 226 of mouse PrP is useful as a diagnostic tool for prion disease of animals.
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
August 2005,
Biomedical and environmental sciences : BES,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
December 2006,
Hybridoma (2005),
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
March 1986,
Shi yan sheng wu xue bao,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
July 2007,
Proceedings of the National Academy of Sciences of the United States of America,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
January 2010,
Cellular immunology,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
November 1995,
Proceedings of the National Academy of Sciences of the United States of America,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
May 2012,
Journal of virology,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
January 1992,
Acta neuropathologica,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
August 1984,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
T Yokoyama, and
K Kimura, and
Y Tagawa, and
N Yuasa
November 2005,
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
