-transparent.png){kind=logo}
Burkholderia cepacia (Pseudomonas cepacia) is now recognised as an important pathogen in cystic fibrosis patients, and several reports have suggested that sputum-culture-proven colonisation occurs despite the presence of specific antibody. In an attempt to establish the use of antibody studies as diagnostic and prognostic indicators of B. cepacia infection, we have examined the IgG response to B. cepacia outer membrane proteins and lipopolysaccharide in patients also colonised with P. aeruginosa. The B. cepacia strains were grown in a modified iron-depleted chemically defined medium and outer membrane components examined by SDS-PAGE and immunoblotting. IgG antibodies were detected against B. cepacia outer membrane antigens, which were not diminished by extensive preadsorption with P. aeruginosa. The response to B. cepacia O-antigen could be readily removed by adsorption of serum either with B. cepacia whole cells or purified LPS, whereas we were unable to adsorb anti-outer membrane protein antibodies using B. cepacia whole cells. The inability to adsorb anti-outer membrane protein antibodies using B. cepacia whole cells maybe due to non-exposed surface epitopes. Several B. cepacia sputum-culture negative patients colonised with P. aeruginosa had antibodies directed against B. cepacia outer membrane protein. this study suggests that there is a specific anti-B. cepacia LPS IgG response, which is not due to antibodies cross-reactive with P. aeruginosa. Our studies indicate that much of the B. cepacia anti-outer membrane protein response is specific and not attributable to reactivity against co-migrating LPS.
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
May 1988,
The Journal of infectious diseases,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
January 1991,
Pediatric pulmonology,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
March 2000,
The Journal of infection,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
August 1999,
The European respiratory journal,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
September 1996,
Microbiological reviews,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
November 1988,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
May 1994,
FEMS immunology and medical microbiology,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
January 1989,
Antibiotics and chemotherapy,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
September 2004,
International journal of medical microbiology : IJMM,
D E Lacy, and
A W Smith, and
D E Stableforth, and
G Smith, and
P H Weller, and
M R Brown
January 2003,
Journal of the Royal Society of Medicine,
