BIOMAT Database Logo BIOMAT Database Logo

Inhibitory effects of Egyptian folk medicines on human immunodeficiency virus (HIV) reverse transcriptase. 1995

S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.

Extracts of 41 medicinal plants used in Egyptian folk medicine were screened for their inhibitory effects on human immunodeficiency virus-1 reverse transcriptase. The extracts of fruits of Phyllanthus emblica, Quercus pedunculata, Rumex cyprius, Terminalia bellerica, Terminalia chebula and Terminalia horrida showed significant inhibitory activity with IC50 < or = 50 micrograms/ml. Through a bioassay guided-fractionation of the methanol extract of the fruit of P. emblica, putranjivain A (1) was isolated as a potent inhibitory substance with IC50 = 3.9 microM, together with 1,6-di-O-galloyl-beta-D-glucose (2), 1-O-galloyl-beta-D-glucose (3), kaempferol-3-O-beta-D-glucoside (4), quercetin-3-O-beta-D-glucoside (5) and digallic acid (6). The inhibitory mode of action by 1, 2 and 6 was non-competitive with respect to the substrate but competitive with respect to a template-primer. Furthermore, the stereochemistry of 1 was established in this paper by nuclear magnetic resonance spectroscopy.

UI MeSH Term Description Entries
D008519 Medicine, Traditional Systems of medicine based on cultural beliefs and practices handed down from generation to generation. The concept includes mystical and magical rituals (SPIRITUAL THERAPIES); PHYTOTHERAPY; and other treatments which may not be explained by modern medicine. Ethnomedicine,Folk Medicine,Folk Remedies,Home Remedies,Medicine, Folk,Medicine, Indigenous,Medicine, Primitive,Indigenous Medicine,Primitive Medicine,Traditional Medicine,Folk Remedy,Home Remedy,Remedies, Folk,Remedies, Home,Remedy, Folk,Remedy, Home
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D010936 Plant Extracts Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D002236 Carbohydrate Conformation The characteristic 3-dimensional shape of a carbohydrate. Carbohydrate Linkage,Carbohydrate Conformations,Carbohydrate Linkages,Conformation, Carbohydrate,Conformations, Carbohydrate,Linkage, Carbohydrate,Linkages, Carbohydrate
D002240 Carbohydrate Sequence The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS. Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D004534 Egypt A country in northern Africa, bordering the Mediterranean Sea, between Libya and the Gaza Strip, and the Red Sea north of Sudan, and includes the Asian Sinai Peninsula Its capital is Cairo. Arab Republic of Egypt,United Arab Republic
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D054303 HIV Reverse Transcriptase A reverse transcriptase encoded by the POL GENE of HIV. It is a heterodimer of 66 kDa and 51 kDa subunits that are derived from a common precursor protein. The heterodimer also includes an RNAse H activity (RIBONUCLEASE H, HUMAN IMMUNODEFICIENCY VIRUS) that plays an essential role the viral replication process. Reverse Transcriptase, HIV,Reverse Transcriptase, Human Immunodeficiency Virus,Transcriptase, HIV Reverse
D018894 Reverse Transcriptase Inhibitors Inhibitors of reverse transcriptase (RNA-DIRECTED DNA POLYMERASE), an enzyme that synthesizes DNA on an RNA template. Reverse Transcriptase Inhibitor,Inhibitors, Reverse Transcriptase,Inhibitor, Reverse Transcriptase,Transcriptase Inhibitor, Reverse

Related Publications

S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Inhibitory effects of triterpenoids and sterols on human immunodeficiency virus-1 reverse transcriptase.
May 2001, Lipids,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Inhibition of human immunodeficiency virus reverse transcriptase by Chinese medicines in vitro.
January 1990, Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Inhibitory effects of antifungal proteins on human immunodeficiency virus type 1 reverse transcriptase, protease and integrase.
January 2002, Life sciences,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Effects of cation substitutions on reverse transcriptase and on human immunodeficiency virus production.
March 1997, AIDS research and human retroviruses,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
[The inhibitory effects of catechin derivatives on the activities of human immunodeficiency virus reverse transcriptase and DNA polymerases].
October 1992, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Human Immunodeficiency Virus type 1 reverse transcriptase.
February 1996, Biological chemistry Hoppe-Seyler,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus.
May 1996, Biochemistry,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Anti-human immunodeficiency virus (anti-HIV) natural products with special emphasis on HIV reverse transcriptase inhibitors.
January 1997, Life sciences,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate on human immunodeficiency virus reverse transcriptase and eukaryotic DNA polymerases.
January 1993, Nucleic acids symposium series,
S el-Mekkawy, and M R Meselhy, and I T Kusumoto, and S Kadota, and M Hattori, and T Namba
A xylanase from roots of sanchi ginseng (Panax notoginseng) with inhibitory effects on human immunodeficiency virus-1 reverse transcriptase.
May 2002, Life sciences,
Copied contents to your clipboard!