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Heart-type fatty acid-binding protein (H-FABP), a 15 kDa non-enzymatic protein, which is abundantly present in heart and some skeletal muscles, was recently found to be a useful plasma marker for acute myocardial infarction. The BIAcore biosensor technology was used to raise and characterize a panel of 13 monoclonal antibodies against human H-FABP which did not crossreact with other FABP types of human origin. The kinetics of association and dissociation between FABP and the monoclonal antibodies was studied. By pairwise mapping several distinct epitopes could be identified.
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